What are some beginner algos that I can use to separate two overlapping nuclei?

I am trying to extract nuclei from images. I have tried WaterShed but it is not very optimal in use. Example images: https://pdfs.semanticscholar.org/8d1f/4a668ffe512499aa7891d1638243fa843bff.pdf

[Jan]

The link does not lead to "example images", but to a publication about segmentation algorithms. It is unlikely that the member of the forum read it and write implement an algorithm for you.

Please explain "not very optimal in use" with any details. You ask for "beginner algorithms". The title of the paper sounds, like you find 2 other algorithms in it also.

[FJ]

Yes yes, I am aware of that and just need push in the right direction. Perhaps I have been unable to utilize watershed to its full potential. The nucleus in this picture attached is not one but 3.

Answer 1

[Image Analyst]

Try Steve's improved watershed method: http://blogs.mathworks.com/steve/2013/11/19/watershed-transform-question-from-tech-support/