measurePSF

Measure a point spread function and other parameters on a microscope
502 Downloads
Aktualisiert 7. Aug 2024

This function measures the full-width-half-max of a microscope point spread function (PSF) along both x and y at the z-plane with the brightest intensity, and also along z/x and z/y. The user simply needs to provide a z-stack containing a PSF (e.g. by imaging a sub-micron fluorescent bead) and also the number of microns per pixel along x/y and z. If using ScanImage (2-photon scanning software) the number of microns per pixel are automatically extracted from the TIFF header.

This submission also contains code and information to measure the number of microns per pixel using an EM grid. If using ScanImage, there is a button in the GUI to apply the measured calibration to ScanImage.

Zitieren als

Rob Campbell (2024). measurePSF (https://github.com/SWC-Advanced-Microscopy/measurePSF), GitHub. Abgerufen.

Kompatibilität der MATLAB-Version
Erstellt mit R2019a
Kompatibel mit allen Versionen
Plattform-Kompatibilität
Windows macOS Linux
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code

code/+mpsf

code/+mpsf/+plot

code/+mpsf/+record

code/+mpsf/+record/private

code/+mpsf/+report

code/+mpsf/+settings

code/+mpsf/+tools

code/+mpsf/+yaml

code/+mpsf/+yaml/Tests

code/+mpsf/+yaml/extras

code/+sibridge

code/@Grid2MicsPerPixel

code/@measurePSF

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Version Veröffentlicht Versionshinweise
2.0.0.0

Update what the submission does.

1.0.0.0

Um Probleme in diesem GitHub Add-On anzuzeigen oder zu melden, besuchen Sie das GitHub Repository.
Um Probleme in diesem GitHub Add-On anzuzeigen oder zu melden, besuchen Sie das GitHub Repository.