how to segment and define regions

Hi all,

This is a phase image reconstructed by NMR.

There are 5 test tubes in this image. One with the largest diameter, and 4 in shorter diameters inside (marked in black). The circular arches are the phase of the molecules.

I need to define the 4 short test tubes and to remove them, but in a way that the new pixels will have a logical value in accordance to it's neighbors.

I will be happy to get a clue how can I manage doing this

Thanks in advance

[Image Analyst]

Are they in the same location all the time? If so, use a template mask. Otherwise, if you need to find them, can you attach the image in a .mat file, or attach the gray scale, non-pseudocolored version?

Also, what does "remove" mean to you? You can't have a hole with missing pixels in an image since images must remain rectangular. However you can assign those pixels to some value, like 0.