How to convert the image into a binary image?

HI, I have a GUI in which I can select multiple regions of interest for an image. I have selected 3 regions in the image. I tried to get the binary image consisting of 3 regions. But unfortunately, I could get only the last(third) region that i selected.

These are the selected regions of interest in the image one by one using the following code.

 global segmentedImage
 if(size(segmentedImage,3)==3)
  im6=rgb2gray(segmentedImage);    
else
  im6=imadjust(segmentedImage);
end

 hFig=figure;
finalImage = segmentedImage;
finalImage(:,:) = 0;
choice = 1; % =2 represents it is done
while(choice == 1)
    % Display the image in Grayscale to draw the contour
  figure;imshow(im6);

    % Enlarge figure to full screen.
  set(gcf, 'units','normalized','outerposition',[0 0 1 1]);
  %  axis on;
  message = sprintf('Draw CONTOUR line.\nLeft click mouse and hold to begin drawing.\nRelease the mouse button to finish');
  uiwait(msgbox(message));
  hFH = imfreehand();
  % Create a binary image ("mask") from the ROI object.
  binaryImage2 = hFH.createMask();    
    subplot(1,2,1);
    imshow(im6);
    title('Original Image','FontSize',fontSize);

    % Get coordinates of the boundary of the freehand drawn region.
    structBoundaries = bwboundaries(binaryImage2);
    xy=structBoundaries{1}; % Get n by 2 array of x,y coordinates.
    x = xy(:, 2); % Columns.
    y = xy(:, 1); % Rows.
    subplot(1, 2, 1); % Plot over original image.
    hold on; % Don't blow away the image.
    plot(x, y, 'LineWidth', 2);
    drawnow;
   
  % Mask the image using bsxfun() function
  maskedImage = bsxfun(@times, im6, cast(binaryImage2, class(im6)));
    imgselected = im2uint8(maskedImage);
  finalImage = imadd(finalImage,imgselected);    
     subplot(1,2,2);
  imshow(maskedImage);
    %  axis on;
  title('Regions to be included', 'FontSize', fontSize);  
  promptMessage = sprintf('Do you want to accept this,\nor draw more regions it?');
  titleBarCaption = 'Continue?';    
    button = questdlg(promptMessage, titleBarCaption, 'Accept', 'More', 'Accept');
        if strcmpi(button, 'More')
           im6=im6-maskedImage;            
        else 
            break;
        end        

        close(gcf);
end
close(hFig);
% End addition

 %figure;imshow(finalBinaryImage);
axes(handles.axes2);
imshow(finalImage);

This is the binary Image that i got using the following code:

 segmentedImageMask=binaryImage-binaryImage2;

Where "binaryImage in the above code" is the binary image of the original image . Here is the attachment of the binary image.

I tried to remove the binary image of the multiple regions(binaryImage2) with the binary image of the original segmented Image(binaryImage). But I couldn't get the appropriate result.

Can you please help me out in getting all the three regions in the binary image.

Thanks...

Answer 1

[Image Analyst]

You need to initialize a binary image and then OR in the ones from the loop. Before the loop

finalBinaryImage = false(size(im6));

Then in the loop

finalBinaryImage  = finalBinaryImage  | binaryImage2;

When the loop is done, finalBinaryImage will have all the regions you drew.

[Manoj Kumar]

When i did this, i could get the first two regions but not all the three regions. Can you please check that. Thanks...

[Image Analyst]

What image do you want as the final output? A gray image with black holes burned into it? A binary image with all the regions you outlined? Three separate binary images?

[Manoj Kumar]

I need a binary image with all the regions outlined...

Answer 2

[Peyman Obeidy]

Greeting I am also interested in doing the same things, did you manage to get all your 3 ROIs on the image. If so, may I get the complete code. This will save me a lot of time.

Cheers Peyman

[Image Analyst]

The key part of the code was this:

message = sprintf('Draw CONTOUR line.\nLeft click mouse and hold to begin drawing.\nRelease the mouse button to finish');
  uiwait(msgbox(message));
  hFH = imfreehand();
  % Create a binary image ("mask") from the ROI object.
  binaryImage2 = hFH.createMask();

[Walter Roberson]

Peyman Obeidy comments to Image Analyst:

were is the start point, I am not use to GUI at all

[Image Analyst]

Peyman, the code is in the original poster's question at the very top. If, some some unreasonable reason, you're unable to use a GUI, then you can't do this at all, because imfreehand() requires a GUI so you can draw on an image.

Answer 3

[Peyman Obeidy]

Dear Image analyser,

what I would like to do is to a- open an image b- select multiple regions c-display selected region on the original image d- calculate the area of each region

for a-b, I found "Multi ROI/Mask Editor Class (https://au.mathworks.com/matlabcentral/fileexchange/31388-multi-roi-mask-editor-class)" GUI, but I can't get the binary and original image as and output into the workspace.

Maybe I get some help with this, I can manage to do the 'c-d' part by myself.