Remove shadow and unwanted background

How can I remove the shadow and extract only the object. I have try imadjust() . But the shadow is still there and during imbinarize()，it will include the shadow..so I cant minus it

Is it possible to make it into the desired picture ?

Before:

Desire

Answer 1

[Joseph Cheng]

to get your started you can see that the tissue and the shadow area have similar values in all the color channels. you can do a bit of comparison of each of the color channels to generate a mask of those which are not gray and white:

img = imread('https://www.mathworks.com/matlabcentral/answers/uploaded_files/645980/image.jpeg');
mtemp = double(img)./median(double(img),3);
    subplot(311),imagesc(mtemp)
    mtemp = mtemp-1;
    subplot(312),imagesc(mtemp)
    mtemp2 = abs(mtemp)<=.30;
    mask = all(mtemp2,3);
    subplot(313),imagesc(mask)

     
     [row col N]=size(img);
     for ind = 1:3
         tempimg = img(:,:,ind);
         tempimg(mask) = 255;
         nimg(:,:,ind) = tempimg;
     end
     
     figure,imagesc(nimg)
     axis equal
    axis tight

which you can use a bit of imerode, bwfill, etc to edit the mask or generate something different.