Using MATLAB with FLIR infrared camera

I am currently using a FLIR E45 handheld infrared camera to capture video images for a research project. I am trying to determine the hottest temperature within the viewing window. Unfortunately there is too much lag time when using the ‘hotspot’ function on the camera in order to accurately assess the hottest point in real time. I’m aware that the E45 camera doesn’t store radiometric data and so I can’t simply use FLIR software to analyze the videos. Can (and how can) MATLAB be used to determine the temperature at the hottest point if the video is an .MP4, using the temperatures already recorded as a reference?