How to save the hold on image? Actually i trace the boundary by the plot under hold on. Now i can't proceed this boundary to further process. As i am in need of this trace boundary "green" lines seperately in the image?

imshow(S)
hold on;
 plot(boundary(:,2),boundary(:,1),'R','LineWidth',3);
 BW_filled = imfill(I,'holes');
boundaries = bwboundaries(BW_filled);
for k=1: length(boundaries)
   b = boundaries{k};
   plot(b(:,2),b(:,1),'G','LineWidth',3);
end