Please help, I was trying to show different graphs with different colors for the improfile, everything that I tried doesn't work, is this even possible? Thank you

I1 = imread([pathname,filename]);

I1 = imrotate(I1,270);

figure;

I2 = imcrop(I1,[1075 174 1000 1649]);

imshow(I2)

hold on

plot([346 884], [566 566],'r-','linewidth',0.1);

plot([884 386], [783 783],'y-','linewidth',0.1);

plot([661 888], [1027 1027],'b-','linewidth',0.1);

x = [346 884];

y = [566 566];

x1 = [386 884];

y1 = [783 783];

x2 = [661 888];

y2 = [1027 1027];

figure;

hold on

improfile(I2,x,y),grid on;

improfile(I2,x1,y1);

improfile(I2,x2,y2);