How can i count the number of cells in an image?

I want to count the number of cells in the given tissue image. Can someone help me with it? I am attaching the image of the tissue.

[Star Strider]

What defines a cell? What is the stain? What parts of the cell is the stain defining?

It looks like a cross-section of a striated muscle with neurovascular bundles and nerve axons, and the lower part looks necrotic.

Answer 1

[Farouk Moukaddem]

Hi Vishakha,

I can see from the picture you uploaded that your image has some background noise. I suggest trying to break the image into a couple of different pieces to separate the main regions of background color. You can do that by using the "imshow" function and then picking specific rows and columns values in the functions "imshow" or "imwrite.

After that, you can work on counting cells that have a more consistent background intensity in each area.

For information about the "imshow" and "imwrite" functions, refer to the following links:

https://www.mathworks.com/help/matlab/ref/imwrite.html

https://www.mathworks.com/help/images/ref/imshow.html

Thanks,

Farouk