Creat a loop for Image processing

Hello, i have the following code, is there any way to rewrite it to create a loop so that it subtracts the 1st photo from 2nd until the 69th and then create a graph as it at the with all the boundaries ?

Thank you very much for the Help guys!

pic01=imread("1.tiff");
pic1=im2gray(pic01);
i=1;
%for i=1:69;
pic050=imread("69.tiff");
pic50=im2gray(pic050);

newpicture=imsubtract(pic01,pic050);
%imshow(newpicture)
level = graythresh(newpicture);
level = 0.1405;
newbinpic = imbinarize(newpicture,level);
%imshowpair(newpicture,newbinpic,'montage')
newbinpic2=bwpropfilt(newbinpic,'perimeter',1);
%imshowpair(newbinpic2,newpicture,'montage'); 
boundaries = bwboundaries(newbinpic2);
binaryImage = 0.6< newbinpic2 & newbinpic2<1;

hold on

for k=1:length(boundaries)
thisBoundary = boundaries{k};
x = thisBoundary(:,2);
y = thisBoundary(:,1);
plot(x,y,'r-','Linewidth',2);
end
grid on

Answer 1

[yanqi liu]

pic01=imread("1.tiff");
pic1=im2gray(pic01);
i=1;
boundaries=[];

for i=1:69;
    pic050=imread("69.tiff");
    pic50=im2gray(pic050);
    
    newpicture=imsubtract(pic01,pic050);
    %imshow(newpicture)
    level = graythresh(newpicture);
    level = 0.1405;
    newbinpic = imbinarize(newpicture,level);
    %imshowpair(newpicture,newbinpic,'montage')
    newbinpic2=bwpropfilt(newbinpic,'perimeter',1);
    %imshowpair(newbinpic2,newpicture,'montage');
    boundariesi = bwboundaries(newbinpic2);
    boundaries=[boundaries; boundariesi];
    binaryImage = 0.6< newbinpic2 & newbinpic2<1;
end

figure; imshow(pic01);
hold on

for k=1:length(boundaries)
    thisBoundary = boundaries{k};
    x = thisBoundary(:,2);
    y = thisBoundary(:,1);
    plot(x,y,'r-','Linewidth',2);
end
grid on

Answer 2

[Matt J]

It is possible:

for i=1:69;
 pic=im2gray( imread(i+".tiff") );
 ...    
end

[Alex Perrakis]

But will it produce a graph with all the boundaries of the images it read?

[Matt J]

If you put the appropriate code in the loop it will.

[Alex Perrakis]

that's what i was asking :) i will have to create a pic variable which runs with the loop right?

[Matt J]

Yes, I've shown you how to create a looping pic variable. The rest of the code you seem to already have in your original post.