Preprocessing

Manage NGS data with single- and paired-end reads, filter and trim reads, and display quality statistics

Preprocess sequence read data using various functions. Filter reads based on the quality of sequencing data and trim termini of sequences. Perform barcode matching (demultiplexing) to group samples based on their barcodes. Split merged paired-end sequence data into two separate files. Calculate quality statistics and visualize such information using quality control plots.

Functions

`seqfilter`	Filter out sequences based on specified criterion
`seqtrim`	Trim sequences based on specified criterion
`seqsplit`	Split sequences into separate files based on barcodes
`seqsplitpe`	Split merged paired-end sequences into separate files
`seqqcplot`	Create quality control plots for sequence and quality data in MATLAB

Topics

Visualize NGS Data Using Genomics Viewer App
View NGS alignment data for single nucleotide variation in cytochrome p450 gene.