I successfully add few .jpg images to few buttons, but when I move to this(GUI with images) GUI from another GUI using
function pushbutton3_Callback(hObject, eventdata, handles)
open('menu_page1.m');
the images I assign to the buttons are not shown,Please help me out..

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Walter Roberson
Walter Roberson am 27 Mai 2012

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open('menu_page1.m') opens the file menu_page1.m in the MATLAB editor, and does not execute the code.
You should either call the code (if it is a function) or run() the code (if it is a script)
menu_page1() %calls a function
run('menu_page1.m') %executes a script

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