Detecting shapes-lsm format?

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Sameer Dixit
Sameer Dixit am 24 Mai 2012
I want to detect the number of blue coloured spots (nuclei to be precise) in an image of lsm format using matlab, however writing the code below is not detecting the shapes at all, instead as i do imshow a white page opens up.(Indicating that the entire picture has been converted into white) code: i=imread('01.lsm'); imshow(i) BW = im2bw(i, 0.9); BW = ~BW; imshow(BW)
Is it a problem with the code or with the format? Thanx in advance.

Antworten (1)

Stephen
Stephen am 24 Mai 2012
try looking at hist(i,100) and picking out a threshold manually. somewhere to the right of the big background spike on the left.
  1 Kommentar
Stephen
Stephen am 24 Mai 2012
oh and imclearborder() bw=bwareaopen() and imfill(bw,'holes') could be pretty useful for you too

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