primer design example - why is scoring matrix changed?
2 Ansichten (letzte 30 Tage)
Ältere Kommentare anzeigen
I need to design long primers of around 50 bp; and i follow the 'Exploring primer design' example (https://se.mathworks.com/help/bioinfo/examples/exploring-primer-design.html). When checking for cross-hybridization, the scoring matrix is changed from NUC44 to scr_mat = [-1,-1,-1,1;-1,-1,1,-1;-1,1,-1,-1;1,-1,-1,-1;]. I have 3 questions: (1) why? what do these numbers mean? (2) are these numbers specific for 18-30 bp, or are they valid for longer oligos? and (3) In this example, 'good' pair is the one with scr <=3, why? what does it mean? Is that 3 or less than 3 nucleotides hybridizing?
0 Kommentare
Antworten (0)
Siehe auch
Kategorien
Mehr zu Bioinformatics Toolbox finden Sie in Help Center und File Exchange
Produkte
Community Treasure Hunt
Find the treasures in MATLAB Central and discover how the community can help you!
Start Hunting!