Color deconvolution for stain separation in biological images

Hello,

I am using the Matlab Image Processing Toolbox to detect cells in biological images.

I have dual-stained images and I would like to apply color deconvolution (as described here: http://www.dentistry.bham.ac.uk/landinig/software/cdeconv/cdeconv.html) to separate the stains. So my input is an RGB image and I would like to get 3 gray scale output images: 1 for each of my stains and 3 third one for everything else (noise). I would then use thresholding on the first two images to detect objects in them.

I have already determined color vectors that describe my 2 stains from sample image regions.

Can anyone give some example code or advice on which functions to use in Matlab to get this done?

Thanks in advance,

Tim

Answer 1

[Manikanta Prasanth]

Hello Tim. If you are still in need of a code, follow this link. http://web.hku.hk/~ccsigma/color-deconv/color-deconv.html

[Walid]

Thanks. I would like to use the algorithm to extract Hematoxylin vs DAB (brown staining). I have separate images with only Hematoxylin or only DAB. How can I do it ?